human mmp-9/timp-1 duoset elisa  (r&d systems)

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: MMP-12 (Human MMP-12 ELISA, Cat # EH327RB, Invitrogen), ADAM-9 (Human ADAM-9 DuoSet ELISA, Cat # DY939, R&D Systems), TIMP-1 (Human TIMP-1 DuoSet ELISA, Cat # DY970, R&D Systems), TIMP-2 (Human TIMP-2 DuoSet ELISA, Cat # DY971, R&D Systems), MMP-9/TIMP-1 complex (Human MMP-9/TIMP-1 DuoSet ELISA, Cat # DY1449, R&D Systems), Cystatin C (Human Cystatin C DuoSet ELISA, Cat # DY1196, R&D Systems), Cathepsin D (Human Cathepsin D DuoSet ELISA, Cat # DY1014, R&D Systems), Serpin E1 (Human Serpin E1 DuoSet ELISA, Cat # DY1786, R&D Systems), Tumor Necrosis Factor alpha (TNF-α) (Human TNF-alpha DuoSet ELISA, Cat # DY210, R&D Systems), IL-1β (Human IL-1 beta DuoSet ELISA, Cat # DY201, R&D Systems), IL-6 (Human IL-6 DuoSet ELISA, Cat # DY206, R&D Systems) and CCL-18 (Human CCL18/PARC DuoSet ELISA, Cat # DY394, R&D Systems) concentrations were measured by sandwich ELISA according to the manufacturer’s protocols.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: Human MMP-9/TIMP-1 DuoSet ELISA , R&D Systems , Cat # DY1449.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: Human TIMP-1 DuoSet ELISA , R&D Systems , Cat #DY970.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry